xdh xo (Bio-Techne corporation)
Structured Review

Xdh Xo, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xdh xo/product/Bio-Techne corporation
Average 92 stars, based on 2 article reviews
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1) Product Images from "Studies on the Effects of Hypercholesterolemia on Mouse Ophthalmic Artery Reactivity"
Article Title: Studies on the Effects of Hypercholesterolemia on Mouse Ophthalmic Artery Reactivity
Journal: Diseases
doi: 10.3390/diseases11040124
Figure Legend Snippet: Specifications of primary antibodies used for immunofluorescence studies.
Techniques Used: Immunofluorescence
Figure Legend Snippet: Messenger RNA expression for the pro-oxidative redox enzymes NOX1, NOX2, NOX4, and XDH/XO in the aorta ( A ) and the ophthalmic artery ( B ) presented as the fold change (mean ± SE) in ApoE−/− mice versus wild-type controls (aorta: n = 8 per genotype; ophthalmic artery: n = 6 per genotype; *** p < 0.001, ApoE−/− versus wild-type mice).
Techniques Used: RNA Expression
Figure Legend Snippet: Immunofluorescence staining for pro-oxidative redox enzymes in aortic tissue sections. Immunoreactivity to NOX1 was markedly elevated in the endothelium of ApoE−/− mice ( A ). Likewise, NOX2 immunoreactivity was pronounced in the ApoE−/− mouse endothelium ( B ). Interestingly, NOX4 immunoreactivity was not elevated in the endothelium, but in the media of ApoE−/− mice ( C ). Immunoreactivity to XDH/XO was weak in both wild-type and ApoE−/− mice ( D ). Data are presented as mean ± SE normalized to wild-type controls (n = 8 per genotype; *** p < 0.001; ** p < 0.01, ApoE−/− versus wild-type mice). The white arrows point to the endothelial layer. Blue: DAPI; Red: Rhodamine Red-X-coupled. Scale bar = 20 µm.
Techniques Used: Immunofluorescence, Staining
Figure Legend Snippet: Immunofluorescence staining for pro-oxidative redox enzymes in ophthalmic artery cross-sections. Immunoreactivity to NOX1 ( A ), NOX2 ( B ), and NOX4 ( C ) was negligible in both wild-type and ApoE−/− mice. Only faint immunoreactivity to XDH/XO was visible in the media and adventitia of the ophthalmic artery but was similar in wild-type and ApoE−/− mice ( D ). Data are presented as mean ± SE normalized to wild-type controls (n = 8 per genotype). The white arrows point to the endothelial layer. Blue: DAPI; Red: Rhodamine Red-X-coupled. Scale bar = 20 µm.
Techniques Used: Immunofluorescence, Staining